Serum Exosomal miRNA Signature in occupational medicamentosa-like dermatitis induced by trichloroethylene

Occupational dermatitis medicamentosa-like of TCE (ODMLT) is a complex immune process which can cause serious damage of liver function, and becoming a serious occupational health problem in China. However, the pathogenesis of ODMLT remained undefined. The aim of the present study was to identify the expression profiles of circulating exosomal miRNAs in ODMLT patients and its role in the regulation of liver injury. The exosomes were isolated from the serum of patients and healthy individuals, and characterized by electron microscopy, nanoparticle tracking analysis, and Western blot and total RNA was extracted. Expression of exosomal miRNAs was evaluated with Agilent Human miRNA microarrays and the expression of selective serum exosomal miRNA were verified by qRT-PCR. Two disease stages (acute-stage and healed-stage) of the 5 ODMLT patients which were ODMLT-A and ODMLT-B group and 5 age- and sex-matched healthy control subjects. The blood was collected into serum-separating tubes and allowed to sit for 30-60min at room temperature prior to centrifugation.