Expression data from mouse plasmacytoid dendritic cells stimulated with CpG and treated with lif
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE138018
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pDCs selectively express the receptor for leukemia inhibitory factor (LIF) that signals through STAT3. We performed microarray analysis of primary pDCs sorted from bone marrow of C57BL6 mice and incubated with a) medium; b) LIF; c) CpG; d) LIF plus CpG. Sorting of pDCs from bone marrow was performed by magnetic beads using negative selection kit (Miltenyi Biotec), followed by sorting of CD11cintB220+SiglecH+ cells by FACS. pDCs were treated with LIF (10ng/ml) for 1 or 24h and stimulated with CpG-A (2216) or CpG-B (1826), both 6ug/ml, for 18-24h.
创建时间:
2020-05-20



