Sequence Data from "Antarctic coastal nanoplankton dynamics revealed by metabarcoding of desalination plant filters: detection of short-term events and implications for routine monitoring"

This dataset includes the original, unmodified demultiplexed fastq files for the submitted paper "Antarctic coastal nanoplankton dynamics revealed by metabarcoding of desalination plant filters: detection of short-term events and implications for routine monitoring". In this paper we have focused on the availability of desalination plant filters in use at the Italian research base (Mario Zucchelli) to monitor coastal plankton communities of the Terra Nova Bay area (Ross Sea). Filters are here used to decrease the amount of organisms and debris in the input seawater before the desalination processes take place, hence they automatically collect the plankton present in the water column around the desalination system intake. Sampling was carried out in January and February of 2012 and 2013 enabling the collection of a total of eleven 5µm cartridge filters (five in 2012, from the 25th of January to the 4th of February, and six in 2013, from the 8th to the 25th of January). Sample names identify the day it each filter was sampled. For example, the filter “30_1_12” was sampled the 30th of January of 2012. These filters, measuring 50.8 cm of length and 6.4 cm of diameter, were kept at -20°C until summer 2018, when they were processed for the molecular analyses. Three replicates were obtained from each filter (one at the top, one in the middle and one at the end of the filter in order to cover all its length), for a total of 33 replicates. PCR amplification and sequencing of the 16S rRNA and 18S rRNA genes, for bacteria and eukaryotes respectively, were performed by IGA Technology (Udine, Italy). The primers used for the V3 and V4 regions of 16S rRNA gene (approximately 450bp) were chosen from Herlemann et al. (2011) and have the following IUPAC codes (comprising the Illumina adapters): 341F - 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG 3’ and 805R - 5’ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC 3’. The primers used for the V4 and V5 regions of the 18S rRNA gene (approximately 550bp) were selected from Hugerth et al. (2012) and have the following sequences (comprising the Illumina adapters): 574*F – 5’ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCGGTAAYTCCAGCTCYV 3’ and 1132R – 5’ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACACCGTCAATTHCTTYAART 3’. Filenames are thus composed of the code "ID1261", the gene name, the microplate position, the original sample name composed of the replicate code (E1, C and E2 for the top, middle and bottom replicate respectively) followed by the date of sampling (e.g. E130112 for the replicate E1 of the filter sampled in January the 30th of 2012), the sample code given by the institution in which the analyses were run, the lane number and the read type (R1 forward, R2 reverse). The sampling date and hours of filtering activity corresponding to each filename are provided in the "sample_info.txt" file.本数据集包含已提交论文《南极海岸纳米浮游生物动态：通过海水淡化厂过滤器的宏条形码检测：短期事件的发现及对常规监测的启示》的原始未修饰的解多路复用fastq文件。本研究聚焦于意大利马里奥·祖切利（Mario Zucchelli）南极科考站在用海水淡化厂过滤器的可用性，以监测特拉诺瓦湾（罗斯海）区域的海岸浮游生物群落。此类过滤器用于在海水淡化流程启动前，减少输入海水中的生物与碎屑含量，因此可自动收集海水淡化系统取水口周围水柱中的浮游生物。采样工作于2012年与2013年的1月至2月开展，共计收集11个5μm规格的筒式过滤器（2012年采集5个，采样时段为1月25日至2月4日；2013年采集6个，采样时段为1月8日至25日）。样本名称以其采样日期命名，例如过滤器“30_1_12”的采样时间为2012年1月30日。此类过滤器长50.8cm、直径6.4cm，在2018年夏季之前一直保存于-20℃环境中，随后被用于分子分析。每个过滤器可获得3个重复样本（分别位于过滤器顶部、中部与末端，以覆盖整个滤柱），总计33个重复样本。由意大利乌迪内的IGA Technology公司完成了针对细菌的16S rRNA基因以及真核生物的18S rRNA基因的PCR扩增与测序。针对16S rRNA基因的V3与V4区域（片段长度约450bp）所用引物选自Herlemann等人2011年的研究，其包含Illumina接头的IUPAC编码如下：341F - 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG 3' 与805R - 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC 3'。针对18S rRNA基因的V4与V5区域（片段长度约550bp）所用引物选自Hugerth等人2012年的研究，其包含Illumina接头的序列如下：574*F – 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCGGTAAYTCCAGCTCYV 3' 与1132R – 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACACCGTCAATTHCTTYAART 3'。文件名由以下部分组成：代码“ID1261”、基因名称、微孔板位置、原始样本名称（由重复样本代码构成：E1、C、E2分别对应顶部、中部、底部重复样本，后接采样日期，例如E130112代表2012年1月30日采样的过滤器的E1重复样本）、分析执行机构提供的样本代码、泳道编号以及读段类型（R1为正向读段，R2为反向读段）。每个文件名对应的采样日期与过滤活动时长已在“sample_info.txt”文件中予以说明。