Identification of MYB/bHLH proteins required for tissue-specific anthocyanin biosynthesis in inner pericarp of red-centered Actinidia chinensis cv. Hongyang

In the present study, we employed the high-throughput sequencing technology to profile miRNAs in the inner and outer pericarp of Actinidia chinensis cv. Hongyang. After sequencing and cleaning, the numbers of clean reads generated from these four libraries were 18,203,332, 9,356,430, 11,006,231 and 11,314,375, respectively. Approximately 93.62%, 93.67%, 92.75% and 93.28% clean reads were respectively mapped to the kiwifruit genome with perfect matches. Subsequently, differentially expressed genes were compared between the inner and outer pericarps. Significant differences in both up- and down-regulated genes were identified in inner and outer percarps by comparing expression levels. The results showed that there were significantly 450 up-regulated and 416 down-regulated DEGs in inner pericarp as compared to the outer pericarp. Overall design: Inner and outer pericarp in two parallel samples of Actinidia chinensis cv. Hongyang