Identification of relevant canonical pathways affected by albumen removal by grouping of the differential expressed proteins through the use of Ingenuity Pathway Analysis (IPA).

IPA-analysis (www.ingenuity.com) was used to identify key biological pathways comprising the differentially identified proteins after prenatal protein undernutrition by albumen removal in chicken. The significance of the canonical pathways was tested by the stringent Benjamini-Hochberg (B-H) multiple testing correction method. The ratio indicates the number of differential proteins in a given pathway divided by the total number of molecules that make up that pathway. The following proteins, annotated with their gene names, are included in the canonical pathways. Abbreviations: ACO2 (Aconitate hydratase); CTH (Cystathionine gamma-lyase); DLD (Dihydrolipoyl dehydrogenase); ENO1 (Alpha-enolase); FBP1 (Fructose-1,6-bisphosphatase 1); GLUD1 (Glutamate dehydrogenase 1); GLUT1 (Solute carrier family 2, facilitated glucose transporter member 1); HIBADH (3-hydroxyisobutyrate dehydrogenase); HSPA2 (Heat shock 70 kDa protein); HSPA9 (Stress-70 protein, mitochondrial precursor); LDHB (L-lactate dehydrogenase B chain); MDH1 (Malate dehydrogenase); PCK2 (Phosphoenolpyruvate carboxykinase); SMAD3 (Mothers against decapentaplegic homolog 3).