<b>T</b><b>etraploid strawberry (</b><b><i>Fragaria moupinensis</i></b><b>)</b>

In order to identify the sex-determining region and gene (s), we sequenced and de novo assembled the female genome of F. moupinensis using 30.80 Gb of PacBio HiFi long reads (34.5X coverage), 151.03 Gb Illumina clean short reads (168.5X coverage), and 43.91 Gb of Hi-C reads (49.1X coverage) . The use of long and accurate HiFi reads coupled with high heterozygosity (5.23%) facilitated the assembly of fully phased haplotypes. This resulted in the generation of four complete haplotypic assemblies named "haplotype A/B/C/D", which were polished for two rounds to produce a tetraploid genome of 896.26 Mb with a contig N50 of 31.68 Mb. Evaluation of k-mers spectra in Smudgeplot revealed a distribution as AAAB (0.58) > AABB (0.13), suggesting the genome of F. moupinensis is autotetraploid . Additionally, the application of Hi-C scaffolding resulted in anchoring 895.13 Mb (99.87%) of the contig sequences to 28 pseudo-chromosomes, with only ten gaps remaining.