Fusarium virguliforme transcriptional plasticity is revealed by host colonization of corn vs. soybean

We exploited the broad host range of Fusarium virguliforme to identify differential fungal responses leading to either an endophytic or a pathogenic lifestyle during colonization of corn (Zea mays) and soybean (Glycine max), respectively. To provide a foundation to survey the transcriptomic landscape, we produced an improved de novo genome assembly and annotation of F. virguliforme using PacBio sequencing. Next, we conducted a high-resolution time course of F. virguliforme colonization and infection of both soybean, a symptomatic host, and corn, an asymptomatic host. Comparative transcriptomic analyses uncovered a nearly complete network rewiring, with less than 8% average gene coexpression module overlap upon colonizing the different plant hosts. Divergence of transcriptomes originating from host specific temporal induction genes is central to infection and colonization, including carbohydrate-active enzymes (CAZymes) and necrosis inducing effectors. Upregulation of Zn(II)-Cys6 transcr...本研究借助弗吉尼亚镰孢菌（Fusarium virguliforme）宽泛的寄主谱，旨在解析其在定殖玉米（Zea mays）与大豆（Glycine max）时，分别走向内生与致病生活模式的差异化真菌应答过程。为搭建转录组全景分析的研究基础，我们利用PacBio测序技术对该菌完成了优化的从头基因组组装与注释。后续，我们针对该菌在感病寄主大豆与无症状寄主玉米中的定殖与侵染过程，开展了高分辨率时间进程实验。比较转录组分析结果显示，该菌在不同植物寄主中定殖时，基因调控网络发生了近乎完全的重编程，基因共表达模块的平均重叠率不足8%。源自寄主特异性时序诱导基因的转录组分化是侵染与定殖的核心调控机制，此类基因涵盖碳水化合物活性酶（CAZymes）与坏死诱导效应因子。锌(II)-Cys6转录（原文未完成）