Transcriptomic, proteomic and phosphoproteomic underpinnings of daily exercise performance and Zeitgeber activity of endurance training in mouse skeletal muscle

Running at different times of the day resulted in a vastly different activation of signaling pathways, e.g., related to stress response, vesicular trafficking, repair, and regeneration. Second, voluntary wheel running at the opposite phase of the dark, feeding period surprisingly revealed minimal Zeitgeber (i.e., synchronizing) activity of training. This integrated study provides important insights into the circadian regulation of endurance performance and the control of the circadian clock by exercise. Investigating the effect of acute treadmill exercise and voluntary chronic wheel running exercise on the transcriptomic, proteomic and phosphoproteomic level at different times of the day in mouse skeletal muscle一日内不同时段进行运动，会引发信号通路激活模式的显著差异，例如与应激反应、囊泡运输、损伤修复及组织再生相关的信号通路。其次，在黑暗、进食周期的相反时段开展自愿跑轮运动时，研究意外发现训练的授时因子（Zeitgeber，即同步因子）活性极低。本整合研究为耐力表现的昼夜节律调控，以及运动对昼夜节律钟的调控机制提供了重要见解。本研究探讨了一日内不同时段下，急性跑步机运动与自愿性慢性跑轮运动对小鼠骨骼肌转录组、蛋白质组及磷酸化蛋白质组水平的影响。