Pairwise RMSD values (in angstroms) between Lmod2s1/αTM1a1-14Zip structures after simulated annealing.

Each simulated annealing structure was produced from one of two starting structures, “model 1” or “model 2.” “Model 1” was a structure with the long (Ala27-Glu38) Lmod2s1 helix parallel to the tropomyosin coiled coil, whereas “model 2” was a structure with the short (Glu18-Ser24) Lmod2s1 helix parallel to the tropomyosin coiled coil. Five replicas (#1–#5) were analyzed for each starting point. To calculate RMSD, backbone atoms N, Cα, and C' of well-structured residues Asp17-Asp39 (Lmod2s1) and Asp2-Leu28 (αTM1a1-14Zip) in two coordinate files were first matched by UCSF Chimera. For the matched structures, a global RMSD (between the same backbone atoms, top table) and a RMSD for the Lmod2s1 peptide (between backbone atoms N, Cα, and C' of residues Asp17-Asp39 in Lmod2s1, bottom table) were calculated. Lmod, leiomodin; RMSD, root-mean-square deviation. (XLSX)