Seq-Scope profiling of mouse colon sections with biopsy-induced injuries

We used Sequence-Scope (Seq-Scope), which can generate ultra-high definition images of sequence-based molecular signatures resolved at a submicrometer scale, for profiling spatial transcriptome associated with biopsy-associated colon injury. Raw 1st-Seq FASTQ with intact tile and coordinate information could be retrieved from https://doi.org/10.5281/zenodo.13118097 The colon samples were obtained from C57BL/6 mice after 24 hrs of biopsy-induced physical wounding. The Seq-Scope procedure was performed on the injured colon sample as previously described with minor modifications involving the use of the Illumina HiSeq 2500 flow cells and chemical strategies to increase RNA sequence capture.