Total RNAseq of intestinal stem cells (ISCs) and progenitor cells (EBs) with expression of Lamin Dm0 and/or UPD

Purpose: To determine the effect of Lamin Dm0 expression on the transcription profile of ISCs/EBs with and without activated Jak/Stat signalling. Method: Lamin Dm0 and UPD were expressed in ISCs/EBs alone and in concert and the transcriptional changes compared with control flies. Conclusions: We found that 49% of UPD induced Jak/Stat targets were normalized by Lamin Dm0 coexpression. Overall design: ISC/EB specific expression of Lamin Dm0 and/or UPD for 5 days. Isolation of ISCs/EBs by FACS and total RNA isolation.