Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to other components of the m6A machinery. Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to other components of the m6A machinery

m6A is catalyzed by the activity of Mettl3, which depends on additional proteins whose precise functions remain poorly understood. Here we identified Zc3h13 as a novel interactor of m6A methyltransferase complex components in mouse. Like other components of this complex, Zc3h13 controls m6A levels. Overall design: We performed m6A-RIP-seq on oligo-dT selected mRNA from wild type, Mettl3-/-, and Zc3h13-/- mESCs. Similar to Mettl3 KO cells, ablation of Zc3h13 resulted in a drastic reduction of m6A enrichment, particularly at the 3’ end of target mRNAs