Maternal Stress During Pregnancy Imprints Fetal Lung Group 2 Innate Lymphoid Cells

We investigated the gene expression on the feral lungs (E18) in the offspring of dexamethasone (DEX)- or PBS- treated mothers by single cell RNA sequence. Fetal lungs were harvested on gestation day 18 from each of two mice that had received water containing DEX or PBS since gestation day 12. Fetal lungs (DEX-1, n = 8; DEX-2, n = 9; PBS-1, n = 5; PBS-2, n = 8) were pooled per sample. Fetal lungs cells were prepared by single-cell suspension protocol of fetal lungs.