Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12176, Gallus gallus gallus, memory B cell. CIL. Dataset
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Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of exogenous TrAP-INCENP (red) plus endogenous CENP-A (green) and DNA (blue) in INCENP OFF cells expressing mutant TrAP-INCENP W766G See Fig 6B panel 13 in Xu et al. 2009 J Cell Biol 187, 637-653.
DT40鸡B淋巴细胞系细胞在有丝分裂阶段的细胞图像,采用宽场免疫荧光技术,通过Deltavision系统搭载100x/1.4NA planapo物镜捕获Z系列图像,并利用约束迭代算法(Applied Precision)进行去卷积处理。展示的是中央的z切片。在INCENP OFF细胞中,外源性的TrAP-INCENP(红色)与内源性的CENP-A(绿色)以及DNA(蓝色)在后期阶段的定位。参见Xu等人在2009年发表在《细胞生物学杂志》(J Cell Biol)第187卷,第637-653页的图6B部分13。
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CIL



