Effects of incineration bottom ash and ammonia on the acidogenic stage of two-stage mesophilic anaerobic digestion

Batch mesophilic 37oC reactors fed with acetic acid (0.5 mL AC/L every 5-6 days), have been amended with different amount of incineration bottom ash/ and ammonium chloride for 120 days. At the start of the experiments, different mass of IBA was added to the reactors which had been amended with IBA. Then the group of the reactors which amended with NH4CL had received 4 g/L NH4Cl every run of 5-6 days. In parallel batch reactors without IBA/ and NH4Cl were also run. Reactor performance (methane production) and stability (pH drop and VFA accumulation) were investigated. On day end of the experiments i.e. on day 120, a representative digestate sample was collected from each reactor, then sequenced for 16S rRNA gene. The sequence files shown in this data set are fastq files from the illumina sequencing analysis.本实验采用序批式中温（37℃）反应器，以乙酸为进料基质，每5~6天按每升反应体系投加0.5 mL乙酸（Acetic Acid, AC），并分别添加不同剂量的焚烧底灰（Incineration Bottom Ash, IBA）与氯化铵，实验周期为120天。实验初始阶段，向预先投加IBA的反应器中加入不同质量的IBA；后续，投加氯化铵的反应器组每5~6天的运行周期内，投加4 g/L的氯化铵。同时设置不添加IBA与氯化铵的平行序批式反应器作为空白对照。本研究考察了反应器的运行性能（产甲烷能力）与运行稳定性（pH下降及挥发性脂肪酸（Volatile Fatty Acid, VFA）积累情况）。于实验结束当日，即第120天时，从每个反应器中采集具有代表性的消化液样品，对其16S rRNA基因（16S ribosomal RNA gene）进行测序。本数据集所包含的测序文件均为Illumina测序分析产出的FASTQ格式文件。