BE-AES:Quantitative Technique Dissecting Nucleic Acid Ionic Atmosphere

Due to the highly charged nature of DNA and RNA, the electrostatic association between ions and nucleic acids predominantly influences the structure and function of the nucleic acid molecules. This project is an experimental technique that can quantitatively and fully dissect the molecular constituents of the ion atmosphere associated with nucleic acids. In this project, buffer equilibration is combined with atomic emission spectroscopy (BE-AES) to read out the number of different monovalent and divalent cations associated with a nucleic acid and to quantitate the relative affinity of the cations. Results establish an unprecedented standard for ion association with nucleic acids and allow rigorous examinations of the widely used Nolinear Poisson-Boltzmann(NLPB) electrostatic theory and other theories (see also ISIM project). <br/><br/>This project includes the following software/data packages: <br/> <ul> <li> <a href="https://simtk.org/frs?group_id=254#pack_350">BE-AES data </a> : download of ion association/depletion with a 24 bp DNA duplex acquired for biologically important monovalent and/or divalent ions </li> </ul>

鉴于DNA与RNA的高度带电性质，离子与核酸之间的静电相互作用主要影响核酸分子的结构和功能。本项目是一种实验技术，能够对与核酸相关的离子气氛的分子组成进行定量和全面的分析。在本项目中，缓冲液平衡与原子发射光谱（BE-AES）相结合，以读取与核酸相关的不同单价和多价阳离子的数量，并定量阳离子的相对亲和力。结果确立了与核酸离子结合的史无前例的标准，并允许对广泛应用的非线性泊松-玻尔兹曼（NLPB）静电理论以及其他理论进行严格的检验（参见ISIM项目）。

本项目包括以下软件/数据包：

<ul>
<li><a href="https://simtk.org/frs?group_id=254#pack_350">BE-AES数据</a>：下载与生物重要单价和多价阳离子相关的24碱基对DNA双链的离子结合/耗竭数据</li>
</ul>