five

Crassocephalum crepidioides accession Ile-Ife HSS cDNA sequences. Crassocephalum crepidioides accession Ile-Ife HSS cDNA sequences

收藏
NIAID Data Ecosystem2026-03-12 收录
下载链接:
https://www.ncbi.nlm.nih.gov/bioproject/PRJEB42680
下载链接
链接失效反馈
官方服务:
资源简介:
The HSS cDNA sequences of Crassocephalum crepidioides accession Ile-Ife were obtained by (I) PCR using redundant primer pairs, (II) 3´- and 5´-RACE and (III) primer pairs allowing amplification of the cDNA. The obtained cDNAs were heterologously expressed in E. coli and enzymatic activity of the experessed homospermidine synthases confirmed by in vitro reactions using spermidine and putrescine as substrates. The formation of homospermidine was confirmed by HPLC analysis.
创建时间:
2021-01-30
二维码
社区交流群
二维码
科研交流群
商业服务