Opsin data from: Multiple axes of visual system diversity in Ithomiini, an ecologically diverse tribe of mimetic butterflies

The striking structural variation seen in arthropod visual systems can be explained by the overall quantity and spatio-temporal structure of light within habitats coupled with developmental and physiological constraints. However, little is currently known about how fine-scale variation in visual structures arise across shorter evolutionary and ecological scales. In this study, we characterise patterns of interspecific (between species), intraspecific (between sexes) and intraindividual (between eye regions) variation in the visual system of four ithomiine butterfly species. These species are part of a diverse 26-Myr-old Neotropical radiation where changes in mimetic colouration are associated with fine-scale shifts in ecology, such as microhabitat preference. By using a combination of selection analyses on visual opsin sequences, in-vivo ophthalmoscopy, micro-computed tomography (micro-CT), immunohistochemistry, confocal microscopy, and neural tracing, we quantify and describe physiolog..., , , # Opsin data from: Multiple axes of visual system diversity in Ithomiini, an ecologically diverse tribe of mimetic butterflies

[https://doi.org/10.5061/dryad.zcrjdfng3](https://doi.org/10.5061/dryad.zcrjdfng3)

Give a brief summary of dataset contents, contextualized in experimental procedures and results.

## Description of the data and file structure

These data consist of a zip folder containing: a) alignment .fasta files for all UV, B, and LW opsin coding sequences used in the selection analyses. Alignments were generated using MACSE v2. and manually cleaned and trimmed with SeaView v5. b) gene tree .treefile files for UV, B, and LW opsins used in the selection analyses, constructed using IQ-TREE (multi-core v1.6.12) using ModelFinder and ultrafast bootstrap.

## Sharing/Access information

All opsin sequences have been uploaded GenBank (accession IDs can be found in Table S1 of Wainwright *et al.* 2023)