The loss of Nudt15 thiopurine detoxification enhances direct DNA damage in hematopoietic stem cells (HSCs). The loss of Nudt15 thiopurine detoxification enhances direct DNA damage in hematopoietic stem cells (HSCs)

The kinetics of HSCs after 6-MP administration were examined by flow cytometry analysis, and genes differentially expressed between HSCs from Nudt15+/+ mice and HSCs from Nudt15R138C/R138C mice were identified by RNA-sequencing before the appearance of 6-MP-induced HSC damage. Overall design: HSCs extracted from Nudt15+/+ and Nudt15R138C/R138C mice treated with 6-MP were analyzed by comparative gene expression profiling using RNA-seq.