Data from: Taxonomic accuracy and complementarity between bulk and eDNA metabarcoding provides an alternative to morphology for biological assessment of freshwater macroinvertebrates

Using broad range universal primers for the DNA barcode marker cox1 (Leray-XT, Wangensteen et al. 2018), we compare community composition captured between morphological and molecular-based approaches from different sources —tissue-based (bulk benthic and bulk drift samples) and environmental DNA (eDNA, filtered water) metabarcoding— for samples collected along a gradient of anthropogenic disturbances. Original Fastq files are deposited in the NCBI SRA repository. The ngsfilter file needed for demultiplexing the fastq files is shared here. Files can be demultiplexed using this ngsfilter table in OBITools ngsfilter command.本研究针对DNA条形码标记基因cox1（cytochrome c oxidase subunit I），采用Leray-XT广谱通用引物（Wangensteen等，2018），对沿人为干扰梯度采集的样本，对比基于形态学与分子生物学两种方法所获取的群落组成。样本来源涵盖两类：基于组织的样本（底栖生物混合样本与漂流物混合样本），以及环境DNA（environmental DNA, eDNA，过滤水样）宏条形码（metabarcoding）样本。 原始FASTQ格式文件已提交至NCBI序列读取档案（Sequence Read Archive, SRA）数据库。用于对FASTQ文件进行序列拆分的ngsfilter配置文件已在此处共享，可通过OBITools工具包中的ngsfilter命令结合该配置表完成文件拆分。