MOESM1 of SOX2OT knockdown derived changes in mitotic regulatory gene network of cancer cells

Additional file 1: Figure S1. The most associated cancers with the SOX2OT long non-coding RNA. The GENEVESTIGATOR tool was used to find out the most related conditions reported in databases to be associated with SOX2OT expression changes. The top significant conditions are listed in left including two types of brain and lung cancers (A). The schematic presentation of the SOX2OT expression level in different tissues adopted from the MiTranscriptome (B). Figure S2. The most similar conditions to the DEGs in SOX2OT knocked down U87-MG cell line (log2 expression). Figure S3. The most similar conditions to the DEGs in SOX2OT knocked down A549 cell line (log2 expression). Figure S4. The heat map presentation of all the significant common DEGs in siRNA treated or control cell line, which red: high expression value, blue: low expression value and white: mean level of expression value. Figure S5. SOX2OT inhibition effect in apoptosis and cell cycle of cancer cell lines. (A) The apoptotic response of the SOX2OTknocked down U-87 MG was compared with control cells with annexin V/PI staining. As is shown, no annexinV-positive cells (FL1) were detected. (B) The flow cytometry evaluation of PI-stained (FL3) cell cycle progression in U-87 MG cell is illustrated in tables for control and SOX2OT knocked down cells. Table S1. The complete common DEGs (P value ≤ 0.05) in both cancer cell lines (A549 and U-87MG). Table S2. Functional gene enrichment results of the common DEGS carried out by Bingo or GeneCodis. Table S3. Functional enrichment of transcription associated genes.