Thermophilic lignocellulolytic microorganisms in composting

This data set contains the information of thermophilic bacteria and fungi isolates obtained from lignocellulose-based composting throughout the critical stages of the process, which have lignocellulose-degrading activity. The collection consists of 186 strains, including 159 bacteria and 27 fungi, for whom the specific composting stage when they were obtained, their quantity and temperature inside the composting pile at sampling time was known. Bacterial strains only had xylanolytic activity while among the fungi also cellulolytic and ligninolytic activities were found for some strains. All isolates were identified by sequencing 16S rRNA or 5.8S-ITS region for bacteria and fungi, respectively. In addition, the range of growth at temperatures between 20 and 60 °C as well as the optimal temperature for growth was determined for each isolate. Each isolate is classified as thermotolerant or thermophilic according to these traits.

本数据集收录了从木质纤维素基堆肥各关键阶段分离得到的、具备木质纤维素降解活性的嗜热细菌与真菌菌株的相关信息。该菌株库共包含186株微生物，其中细菌159株、真菌27株；所有菌株分离时所处的堆肥阶段、采样时的菌株数量及堆肥堆内部温度均已记录。细菌菌株仅表现出木聚糖降解活性，而部分真菌菌株同时具备纤维素降解与木质素降解活性。所有分离菌株均通过测序完成鉴定：细菌菌株采用16S rRNA测序，真菌菌株采用5.8S-ITS区域测序。此外，本研究测定了每株菌株在20℃至60℃温度区间内的生长情况，以及其生长最适温度。依据上述测定特性，每株菌株被归类为耐热菌（thermotolerant）或嗜热菌（thermophilic）。