ChIP-seq profiling of PU.1 binding sites in plasmacytoid and conventional mouse dendritic cells

Dendritic cells (DCs) are can be broadly divided into conventional (cDC) and plasmacytoid (pDC) subsets. Despite the importance of this lineage diversity, its genetic basis is not fully understood. ChIP-seq profiling of PU.1 binding sites in cDCs and pDCs revealed a key role for PU.1 in maintaining cDC identity. ChIP-seq was conducted for two biological replicates of cDCs and pDCs with a PU.1 antibody. Sequencing was undertaken at the Beijing Genomic Institute (BGI) on an Illumina HiSeq 2000 to generate 49 base-pair single-end reads.