Melioidosis DS assay results.

Melioidosis DS assay signal intensities for the four spotted antigens tested with melioidosis positive sera and controls. Signal intensities were obtained by comparison of the respective band intensities to the gold reference card (Senova, Germany). Melioidosis DS assays were evaluated independently by three individuals. Furthermore, a binary representation (positive—“1”/negative—“0”) is shown for each band besides the overall number of positive bands per assay. Finally, the Melioidosis DS assay result is shown, for two conditions: (1) at least one positive band, (2) at least two positive bands. Furthermore, corresponding protein microarray and IHA results for the used sera are included for comparison. Signals of at least two antigens have to be higher than the array threshold of 0.3 for protein microarrays to be considered positive. IHA result lower than 160 were regarded negative. (XLSX)