sRNA and transcriptome sequencing of populus simonii × P. nigra

Cold tolerance is important for plants geographical distribution and surviving in extreme seasonal variations of climate. However, populus simonii × P. nigra shows wide adaptability and strong cold resistance. Transcriptional and post-transcriptional regulation of cold-responsive genes is crucial for cold tolerance in plants. To understand the roles of regulatory RNAs under cold stress in populus simonii × P. nigra, we constructed cDNA and small RNA libraries from leaf buds treated or not with 4 °C for 8 h for analysis.