Deep-sea sediment Microtox toxicity data analyzed from sediment cores collected aboard multiple research cruises in the northern Gulf of Mexico from 2010-09-16 to 2010-10-23

This dataset contains deep-sea sediment Microtox toxicity data analyzed from sediment cores collected aboard R/V Ryan Chouest (10-07-10-RC, 2010-10-07 to 2010-10-17), R/V Ocean Veritas (09-24-2010_Ocean_Veritas, 2010-09-24 to 2010-10-23) and R/V Gyre (09-14-2010_Gyre, 2010-09-14 to 2010-10-20) cruises in the northern Gulf of Mexico from 2010-09-16 to 2010-10-23. Following the Deepwater Horizon oil spill, a series of cruises was undertaken to determine if oil reached the bottom and if sediment toxicity was occurring. Sampling occurred after the wellhead was capped from September through October 2010. Sediment sampling was performed using OSIL multiple corers at 179 stations at water depths ranging from 16 m to 2767 m. A sediment quality approach was used where chemical contaminants were measured to indicate a dose effect, and in situ bacterial toxicity to indicate a biological effect. Three sediment sub-cores were taken from one multi-corer core using a 10 cc (=10 ml) syringe from the undisturbed surface. The three sub-cores were composited into one 30 cc sediment sample for toxicity analysis. Some sediments were analyzed immediately on shipboard, but other sediments were preserved at 20 ºC and analyzed on-shore. Samples measured on shipboard were held at 4 ºC in the dark. The bacteria preparation is added to bulk sediment samples, mixed thoroughly, and then a dilution series is created, and the samples are exposed for 30 minutes. The bacteria and sediment are pelleted, and fluorescence measurements are taken to determine the concentration that provides an EC50 response. The dataset contains the depth, date, and location of sampling collection stations, effect concentration 50 values, and the total concentration of polycyclic aromatic hydrocarbons (PAHs).本数据集包含墨西哥湾北部海域2010年9月16日至10月23日期间的深海沉积物Microtox毒性数据，数据来源于“瑞安·舒斯特号”（R/V Ryan Chouest，航次编号10-07-10-RC，2010年10月7日至17日）、“海洋真理号”（R/V Ocean Veritas，航次编号09-24-2010_Ocean_Veritas，2010年9月24日至10月23日）及“旋回号”（R/V Gyre，航次编号09-14-2010_Gyre，2010年9月14日至10月20日）三场科考航次采集的沉积岩芯分析结果。 深水地平线（Deepwater Horizon）漏油事件发生后，科研团队开展了一系列科考航次，以探明海底是否遭受原油污染，以及是否存在沉积物毒性问题。 本次采样作业于2010年9月至10月井口封堵后进行。 采样过程采用OSIL多管沉积物采样器，在179个站位开展沉积物取样，作业水深范围为16米至2767米。 本研究采用沉积物质量评估方法，通过测定化学污染物含量以表征剂量效应，同时开展原位细菌毒性实验以反映生物效应。 从单根多管采样岩芯的未扰动表层，使用10 cc（即10 ml）注射器采集3份沉积物亚岩芯。 将3份亚岩芯混合为一份30 cc的沉积物样品，用于毒性分析。 部分沉积物样品于船上即时开展分析，其余样品则于20℃下保存并在陆地实验室完成检测。 船上待测样品于4℃避光条件下储存。 将细菌制剂与整体沉积物样品充分混匀后制备梯度稀释液，将样品与细菌共同孵育30分钟。 随后离心分离细菌与沉积物，通过荧光光度测定获取半数效应浓度（EC50）。 本数据集包含采样站位的水深、采样日期与地理位置、半数效应浓度值以及多环芳烃（polycyclic aromatic hydrocarbons，PAHs）总浓度。