Next Generation Sequencing Facilitates Quantitative Analysis of young and aged control and Acsl6-/- Transcriptomes

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived cerebellar transcriptome profiling (RNA-seq) to determine age-related impact of acsl6 deficiency on brain transcriptome profiles. Methods: Cerebellar mRNA profiles of 2-month and 18-month control and long-chain acyl-coa synthetase 6-knockout (Acsl6) mice were generated and sent to Genewiz for RNAseq analysis Results: Using an optimized data analysis workflow, we mapped about 3,451,694,754 sequence reads total and ~25-45 million reads per sample of which ~98% were mapped to the mouse genome. Conclusions: Our study represents the first detailed analysis of cerebellar transcriptomes of Acsl6 knockout mice, compared to controls, at young and old ages. Cerebelum poly-A mRNA profiles of 2-month and 18-month old control and Acsl6-/- mice.