Phylogeny of Mycoplasma bovis isolates from Hungary based on multi locus sequence typing and multiple-locus variable-number tandem repeat analysis

Background: Mycoplasma bovis is an important pathogen causing pneumonia, mastitis and arthritis in cattle worldwide. As this agent is primarily transmitted by direct contact and spread through animal movements, efficient genotyping systems are essential for the monitoring of the disease and for epidemiological investigations. The aim of this study was to compare and evaluate the multi locus sequence typing (MLST) and the multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) through the genetic characterization of M. bovis isolates from Hungary. Results: Thirty one Hungarian M. bovis isolates grouped into two clades by MLST. Two strains had the same sequence type (ST) as reference strain PG45, while the other twenty nine Hungarian isolates formed a novel clade comprising five subclades. Isolates originating from the same herds had the same STs except for one case. The same isolates formed two main clades and several subclades and branches by MLVA. One clade contained the re...背景：牛支原体（Mycoplasma bovis）是一种在全球范围内引发牛肺炎、乳腺炎及关节炎的重要致病性病原。该病原体主要通过直接接触传播，并随动物流动扩散，因此高效的基因分型系统对于该病的监测及流行病学调查至关重要。本研究通过对匈牙利来源的牛支原体分离株进行遗传特征分析，旨在比较并评估多位点序列分型（multi locus sequence typing, MLST）与多位点可变数目串联重复序列（variable-number tandem-repeat, VNTR）分析（MLVA）两种分型方法。 结果：经MLST分型，31株匈牙利牛支原体分离株可分为两个进化枝。其中2株的序列型（sequence type, ST）与参考株PG45一致，其余29株匈牙利分离株则构成一个包含5个亚进化枝的全新进化枝。除1例外，同一牛群来源的分离株具有相同的序列型（ST）。采用MLVA分型时，该批分离株可形成2个主要进化枝及若干亚进化枝与分支。其中一个进化枝包含re...