Stimulation of gliding motility depends on TraA.

(A) Indicated motility phenotypes shown for reference. To easily observe stimulation the donor strain (DK6204) was nonmotile (A−S−), i.e. produces sharp colony edges, and nonstimulatable, while the (B) cgl and tgl recipients were phenotypically A−S− but stimulatable. (C) Indicated mutants mixed 1∶1 with the donor exhibiting various degrees of A- or S-motility stimulation. (D) Same as C, except strains mixed with isogenic traA::km donor. (E) Same as C, except isogenic recipient strains contain the traA::km mutation. Cells were incubated for 22 hrs at 33°C and observed with 10× objective. Table S2 lists strain genotypes.