Single cell RNA sequencing of normal thyroid

We performed single-cell RNA-sequencing of 54,762 cells derived from normal thyroid tissue from 7 patients, revealing distinct cell types including epithelial cells, fibroblasts, endothelial cells, smooth muscle cells/pericytes, and various immune cells. Single-cell RNA-seq data were mapped, and counts of molecules per barcode were quantified using the 10x software package cellranger (version 3.0.2) and GRCh38 reference genome supplied by 10x Genomics. To exclude low quality or doublets, we performed Scrublet and filtered cells with fewer than 200 genes or cells with 25% or higher mitochondrial proportion and obtained 54,726 cells from 7 normal thyroid samples. **To ensure preservation of patient confidentiality, raw data are available on request.**