Expression data from ELC52 cells

Microarray analysis showed that TPO addition induced mRNA expression of chemokine families in ELC52 cells, and ruxolitinib treatment reduced mRNA expression of certain chemokine families in ELC52 cells. IFN-α inhibited ELC52 cell proliferation, and this was accompanied by induction of IFN-stimulated genes. Total RNA from treated ELC52 cells was isolated using a RNeasy Plus Mini Kit (QIAGEN). Total RNA was subjected to human Clarion S assay (Applied Biosystems). Reagent concentrations were DMSO (0.01%), ruxolitinib (1 microM), TPO (100 ng/ml), and IFNalpha2b (200 ng/ml).