Genome-wide miRNA expression analysis of Caenorhabditis elegans AXIN mutant

Purpose: The goal of this study is to identify differentially expressed microRNAs in pry-1/Axin mutant compare to N2 wild-type (WT).  Our study represents the first microRNA analysis of Axin transcriptome in C. elegans and facilitates investigations of axin mediated processes. Overall design: Whole animal total RNA was extracted from L1 synchronized worms and total small RNA profiles of WT and pry-1(mu38) animals were generated by single end deep sequencing, using Illumina HISeq 2000. The sequence reads that passed quality filters were analyzed using mirbase cel.gff3 with mirDeep2 star to estimate transcript abundances and differentially-expressed microRNAs at a false discovery rate (FDR) of 0.05%.