Data from: HIV-1 Vif disrupts phosphatase feedback regulation at the kinetochore, leading to a pronounced pseudo-metaphase arrest

This dataset supports the following findings: the HIV-1 Virion Infectivity
Factor (Vif) promotes viral infection by targeting and degrading cellular
APOBEC3 proteins, which are key regulators of intrinsic and innate
antiviral immunity. Although Vif is also known to induce cell cycle
arrest, its precise effects on the cell cycle have remained unclear. Using
high-temporal-resolution single-cell live imaging and super-resolution
microscopy, we found that Vif does not disrupt the G2/M boundary as
previously proposed. Instead, Vif induces a distinct and robust
pseudo-metaphase arrest, unlike the mild prometaphase arrest caused by
Vpr. During this arrest, chromosomes initially align at the metaphase
plate but later lose alignment, producing polar chromosomes. Notably, Vif
markedly reduces Protein Phosphatase 1 (PP1) and Protein Phosphatase 2A
(PP2A) levels at kinetochores, unlike Vpr. These findings reveal a novel
role for Vif in kinetochore regulation and chromosome organization during
mitosis.