Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes determined by activity-based chemical trapping strategy (adjacent H2AK13/15 dual-monoubiquitination)

Cryo-EM structures of RNF168/UbcH5c-Ub in complex with H2AK13Ub nucleosomes determined by activity-based chemical trapping strategy (adjacent H2AK13/15 dual-monoubiquitination) Descriptor: DNA (143-MER), E3 ubiquitin-protein ligase RNF168, Histone H2A type 1-B/E, ... Authors: Ai, H.S, Tong, Z.B, Deng, Z.H, Pan, M, Liu, L. Deposit date: 2023-11-24 Release date: 2024-08-07 Last modified: 2025-06-25 Method: ELECTRON MICROSCOPY (3.27 Å) Cite: Capturing Snapshots of Nucleosomal H2A K13/K15 Ubiquitination Mediated by the Monomeric E3 Ligase RNF168 Biorxiv, 2024通过基于活性的化学捕获策略解析的RNF168/UbcH5c-Ub与H2AK13Ub核小体复合物的冷冻电镜（Cryo-EM）结构（该复合物带有相邻H2AK13/15双单泛素化修饰） 描述符：DNA（143聚体）、E3泛素蛋白连接酶RNF168、组蛋白H2A 1-B/E型…… 作者：Ai, H.S、Tong, Z.B、Deng, Z.H、Pan, M、Liu, L. 存入日期：2023-11-24 发布日期：2024-08-07 最后修改日期：2025-06-25 实验方法：电子显微镜（分辨率3.27埃） 引用文献：《捕获单体E3连接酶RNF168介导的核小体H2A K13/K15泛素化快照》，BioRxiv，2024