Figure 5d

Fluorescence confocal images of lineage-depleted iWAT SVF from Bbs8+/+ and Bbs8-/- mice, isolated at 6-8 weeks (lean state), labeled against (c) Smoothened (green, SMO) or (d) GPR161 (green), and ARL13B (red, cilia), g-Tubulin (magenta, basal body), and with DAPI (blue). Cells were treated with H2O (control) or 1 µM SAG for 24 h. Scale bars are indicated. In all images, the green channel (SMO or GPR161, respectively) was shifted by 10 px to the bottom for better visualizing SMO or GPR161 accumulation in cilia.