Information S1 - Increased Virulence of an Epidemic Strain of Mycobacterium massiliense in Mice

(A) Partial sequencing of the hsp65 and rpoB genes of M. massiliense CIP 108297 and CRM-0019. The corresponding sequences of M. abscessus ATCC 19977 are included in the alignments as a reference. PCR amplification were performed as described by Ringuet et al. (1999) and Kirshner et al. (1993) (see references below). (B) Growth rates of M. massiliense CIP 108297 and CRM-0019 in 7H9-OADC-Tween 80 broth at 30°C. M. massiliense CIP 108297 (diamonds); M. massiliense CRM-0019 (rectangles). (C) GTA susceptibility of M. massiliense CIP 108297 and CRM-0019. Results are expressed as CFU counts upon exposure of the test organisms to 2.2% GTA (under the formulated form of Cidex®, Johnson & Johnson) for 0 to 30 min. M. massiliense CIP 108297 (diamonds); M. massiliense CRM-0019 (rectangles). (PDF)