Affinity purification of biotinylated AtACINUS and Mass Spectrometric analysis to identify PTMs on AtACINUS
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https://www.omicsdi.org/dataset/pride/PXD020749
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AtACINUS protein is involved in regulation of alternative transcription and splicing (AS). Previously we have identified O-GlcNAcylation and phosphorylation sites on AtACINUS using LWAC and IMAC enrichment (Xu et al., 2017). To identify more modification sites, we have created AtACINUS-YFP-TurboID/acinus-2pinin transgenic lines and pulled down AtACINUS using streptavidin beads to identify post-translational modifications on AtACINUS.
创建时间:
2020-12-02



