mir-284-dependent dynamics of the Drosophila antennal transcriptome during aging

We isolated mir-284 from a genetic screen for age-dependent axon degeneration in Drosophila melanogaster. mir-284 levels show a steep decline with aging. Therefore we embarked on a transcriptome-wide analysis after mir-284 depletion in aging flies. To shed light on the mir-284-regulated transcriptome, we performed RNA sequencing (RNA-seq) on wild-type (w1118) and mir-284 KO (w1118;;mir-284 KO) antennae at 3 and 25 days of age in three biological replicates (2 biological replicates for mir-284 KO at 25d) on an Illumina NextSeq.