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Data from: Resources for phylogenomic analyses of Australian terrestrial vertebrates

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DataONE2016-11-07 更新2024-06-26 收录
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High throughput sequencing methods promise to improve our ability to infer the evolutionary histories of lineages and to delimit species. These are exciting prospects for the study of Australian vertebrates, a group comprised of many globally unique lineages with a long history of isolation. The evolutionary relationships within many of these lineages have been difficult to resolve with small numbers of loci and we now know that many lineages also exhibit substantial cryptic diversity. Here, we present a set of phylogenetically diverse transcriptome resources to enable exon-based sequence capture studies of Australian vertebrates, including transcriptome sequences for 4 species of birds, 4 frogs, 7 lizards and 7 mammals. We also use exon data from the marsupial transcriptomes we generated to examine an approach for choosing a moderate number (dozens or hundreds) of phylogenetically informative exons based on a single transcriptome sequence, and a relatively distant reference genome.

高通量测序(High throughput sequencing)技术有望提升我们推断演化支系历史、开展物种界定的能力。这为澳大利亚脊椎动物的研究带来了令人振奋的前景——该类群包含诸多全球特有支系,且拥有漫长的隔离演化历史。此前,借助少量基因座(loci)难以解析诸多这类支系内部的演化关系,现已证实,诸多支系还存在显著的隐存多样性。本研究构建了一组系统发育多样性丰富的转录组(transcriptome)资源,以支撑澳大利亚脊椎动物的外显子靶向序列捕获研究,其中包含4种鸟类、4种蛙类、7种蜥蜴及7种哺乳类的转录组序列。此外,我们还利用所获得的有袋类转录组的外显子数据,检验了一种基于单份转录组序列与相对远缘参考基因组,筛选适量(数十至数百个)系统发育信息外显子的方法。

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2016-11-07
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