Competent B. subtilis cells auxotrophic for methionine were transformed with chromosomal DNA from a met+ (SB19) strain. The yield of met+ transformants (chromosomal transformation), kanamycin-resistan

NIAID Data Ecosystem40

Integration assay expressing trwC +/− trwA in the recipient cell.

细菌接合转移

质粒转化效率

Donor cells harbouring the suicide mobilizable plasmid pR6K::oriTP oriTw were either S17.1 λpir for TraI-mediated mobilization, or II1 (pCIG1077) for TrwC-mediated mobilization. Recipient strain was D

NIAID Data Ecosystem20

Transformation events.

植物遗传转化

质粒转化效率

Number of independent transformation events, regenerated embryos, positively transformed plantlets and transformation percentage per batch measured for four plasmids (1, 2, 3 and 4 for pKGW-RR, pG-Rec

Figshare2022-02-03 更新40

Transformation frequencies of Escherichia coli strain TB1 for pSiM24 binary vector.

质粒转化效率

微生物遗传操作

The bacteria were transformed with equal molar amounts of each of the plasmid DNA. Statistical analysis of the data was performed adopting one way ANOVA analysis (using GraphPad Prism version 5.01) an

NIAID Data Ecosystem30

Conjugation efficiency of pLE variants.

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