Analysis of vastus lateralis muscle from 30 human subjects classified by metabolic flexibility

To determine the transcriptomic effects on skeletal muscle gene expression between metabolically flexible and inflexible subjects, we assessed vastus lateralis muscle from 15 males and 15 females using a serial analysis of gene expression strategy. Complementary bioinformatics pathway enrichment studies were performed which indicated that differentially expressed genes between genotypes were predominantly related to various processes include carbohydrate metabolism, lipid metabolism, amino acid metabolism, cell proliferation, and apoptosis. Vastus laterlis muscles were taken from 30 human subjects classified as either metabolically flexible or inflexible based upon differences between 24hr and sleep respiratory quotient. Total RNA was prepared using Trizol, RNA concentration was determined usign a nanodrop UV spectrophotometer, and 500ng RNA per sample was used for sequencing library construction.