Data from: Pathogen exposure disrupts an organism’s ability to cope with thermal stress

When using this data, please cite the original publication:Hector, T.H., Sgrò C. and M. D. Hall (2019) Pathogen exposure disrupts an organism's ability to cope with thermal stress. Global Change Biology. doi: 10.1111/gcb.14713Files in this package:Title: hector_et_al_experiment1_heatshock_data.csvDescription: Data from experiment 1 heatshock assay assessing knockdown times at 37 degrees. File contains: host_gen = host genotype ("H" & "M"),pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), assay_run = assay replicate date, knockdown_time_mins = individual knockdown times (mins)Title: hector_et_al_experiment1_ramp_data.csvDescription: Data from experiment 1 ramp assays assessing ctmax across different temperature ramping rates. File contains: host_gen = host genotype ("H" & "M"), pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), assay_run = assay replicate date, ramping_rate = assay temperature ramping rate (0.06, 0.04 and 0.02 degrees per minute), ctmax = individual CTmax (degrees celsius).Title: hector_et_al_experiment2_heatshock_full_data.csvDescription: Data from experiment 2 heatshock assay assessing knockdown times across stages of infection (days post infection). File contains: host_gen = host genotype ("H" & "M"), pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), assay_run = assay replicate, dpi_fct = days post infection ("10", "20", "30", "40" & "50), knockdown_time_mins = individual knockdown times (mins)Title: hector_et_al_experiment2_ramp_full_data.csvDescription: Data from experiment 2 ramp assay assessing ctmax across stages of infection (days post infection).File contains: host_gen = host genotype ("H" & "M"), pathogen_gen = pathogen genotype ("C1", "C14" & "C20" & uninfected "CTRL"), assay_run = assay replicate, dpi_fct = days post infection ("10", "20", "30", "40" & "50),ctmax = individual CTmax (degrees celsius).Title: hector_et_al_experiment2_heatshock_data_infected.csvDescription: Data from experiment 2 heatshock assay used for analysing how spore loads, relative host body size and days post infection impact relative knockdown times (relative to uninfected controls). File contains:host_gen = host genotype ("H" & "M")pathogen_gen = pathogen genotype ("C1", "C14" & "C20") assay_run = assay replicatedpi_fct = days post infection ("20", "30", "40" & "50), body_size = raw individual body sizeknockdown_time_mins = raw individual knockdown timesctrl_time = mean control knockdown times for each host genotype by dpi combinationctrl_size = mean control body sizerelative_time = knockdown times relative to corresponding control meanrelative_size = body size relative to corresponding control meanmature_spores_mil = individual pathogen spore counts (millions)Title: hector_et_al_experiment2_ramp_data_infected.csvDescription: Data from experiment 2 ramp assay used for analysing how spore loads, relative host body size and days post infection impact relative CTmax (relative to uninfected controls). File contains:host_gen = host genotype ("H" & "M")pathogen_gen = pathogen genotype ("C1", "C14" & "C20") assay_run dpi_fct = days post infection ("20", "30", "40" & "50), body_size = raw individual body sizeknockdown_time_mins = raw individual knockdown timesctrl_time = mean control knockdown times for each host genotype by dpi combinationctrl_size = mean control body sizerelative_ctmax = ctmax relative to corresponding control meanrelative_size = body size relative to corresponding control meanmature_spores_mil = individual pathogen spore counts (millions)

使用本数据集时，请引用原始文献：Hector, T.H.、Sgrò C. 与 M. D. Hall（2019）发表于《Global Change Biology》的论文《Pathogen exposure disrupts an organism's ability to cope with thermal stress》，DOI: 10.1111/gcb.14713。

本数据包内包含以下文件：
1. **hector_et_al_experiment1_heatshock_data.csv**
标题：实验1热激击倒试验数据
描述：本数据集来自实验1的热激测定试验，用于评估37℃条件下的个体击倒时间。文件包含字段：宿主基因型（host_gen，取值为“H”与“M”）、病原体基因型（pathogen_gen，取值为“C1”、“C14”、“C20”以及未感染对照组“CTRL”）、试验重复日期（assay_run）、个体击倒时间（knockdown_time_mins，单位：分钟）。
2. **hector_et_al_experiment1_ramp_data.csv**
标题：实验1升温速率试验数据
描述：本数据集来自实验1的升温试验，用于评估不同升温速率下的个体临界高温（CTmax）。文件包含字段：宿主基因型（host_gen，取值为“H”与“M”）、病原体基因型（pathogen_gen，取值为“C1”、“C14”、“C20”以及未感染对照组“CTRL”）、试验重复日期（assay_run）、升温速率（ramping_rate，单位：℃/分钟，取值为0.06、0.04与0.02）、个体临界高温（ctmax，单位：摄氏度）。
3. **hector_et_al_experiment2_heatshock_full_data.csv**
标题：实验2完整热激击倒试验数据
描述：本数据集来自实验2的热激测定试验，用于评估不同感染后天数（dpi，days post infection）下的个体击倒时间。文件包含字段：宿主基因型（host_gen，取值为“H”与“M”）、病原体基因型（pathogen_gen，取值为“C1”、“C14”、“C20”以及未感染对照组“CTRL”）、试验重复（assay_run）、感染后天数（dpi_fct，取值为“10”、“20”、“30”、“40”与“50”）、个体击倒时间（knockdown_time_mins，单位：分钟）。
4. **hector_et_al_experiment2_ramp_full_data.csv**
标题：实验2完整升温速率试验数据
描述：本数据集来自实验2的升温试验，用于评估不同感染后天数（dpi）下的个体临界高温（CTmax）。文件包含字段：宿主基因型（host_gen，取值为“H”与“M”）、病原体基因型（pathogen_gen，取值为“C1”、“C14”、“C20”以及未感染对照组“CTRL”）、试验重复（assay_run）、感染后天数（dpi_fct，取值为“10”、“20”、“30”、“40”与“50”）、个体临界高温（ctmax，单位：摄氏度）。
5. **hector_et_al_experiment2_heatshock_data_infected.csv**
标题：实验2感染组热激击倒试验数据
描述：本数据集来自实验2的热激测定试验，用于分析病原体孢子负载量、宿主相对体型以及感染后天数对相对击倒时间（相对于未感染对照组）的影响。文件包含字段：宿主基因型（host_gen，取值为“H”与“M”）、病原体基因型（pathogen_gen，取值为“C1”、“C14”与“C20”）、试验重复（assay_run）、感染后天数（dpi_fct，取值为“20”、“30”、“40”与“50”）、个体原始体型（body_size）、个体原始击倒时间（knockdown_time_mins，单位：分钟）、各宿主基因型与感染后天数组合对应的对照组平均击倒时间（ctrl_time）、各宿主基因型与感染后天数组合对应的对照组平均体型（ctrl_size）、相对于对应对照组均值的相对击倒时间（relative_time）、相对于对应对照组均值的相对体型（relative_size）、个体病原体孢子计数（mature_spores_mil，单位：百万）。
6. **hector_et_al_experiment2_ramp_data_infected.csv**
标题：实验2感染组升温速率试验数据
描述：本数据集来自实验2的升温试验，用于分析病原体孢子负载量、宿主相对体型以及感染后天数对相对临界高温（CTmax，相对于未感染对照组）的影响。文件包含字段：宿主基因型（host_gen，取值为“H”与“M”）、病原体基因型（pathogen_gen，取值为“C1”、“C14”与“C20”）、试验重复（assay_run）、感染后天数（dpi_fct，取值为“20”、“30”、“40”与“50”）、个体原始体型（body_size）、个体原始击倒时间（knockdown_time_mins，单位：分钟）、各宿主基因型与感染后天数组合对应的对照组平均击倒时间（ctrl_time）、各宿主基因型与感染后天数组合对应的对照组平均体型（ctrl_size）、相对于对应对照组均值的相对临界高温（relative_ctmax）、相对于对应对照组均值的相对体型（relative_size）、个体病原体孢子计数（mature_spores_mil，单位：百万）。