Jennifer Bestman, Holly Cline (2011) CIL:36967, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #1 (0 hour) of a time lapse series contained with in this image group. It shows neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.

The images were acquired at 4 hour intervals over 8 hours, and show morphological plasticity of the dendritic arbor with branch additions and retractions.

These cells were transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

本图组中包含的延时摄影系列图像之一，编号为图像 #1（0小时）。图像展示了无色斑马鱼Xenopus laevis蝌蚪中枢神经系统视顶盖中的神经元，处于第47发育阶段。这些图像每隔4小时采集一次，历时8小时，展现了树突丛的形态可塑性，包括分支的增加与回缩。这些细胞在采集第一张图像前约24小时，通过体内电穿孔转染了编码eYFP的质粒DNA。完整的活体蝌蚪经过麻醉，置于载玻片下，并通过定制构建的双光子显微镜直接穿透其透明皮肤进行成像。