Transcriptome analysis of chrysanthemum (Dendranthema grandiflorum) in response to low temperature stress

We used the RNA-Seq platform to perform a de novo transcriptome assembly to analyze chrysanthemum (Dendranthema grandiflorum) transcription response to low temperature. Using Illumina sequencing technology, a total of 86,444,237 high-quality clean reads and 93,837 unigenes were generated from four libraries: T01, controls; T02, 4 ? cold acclimation (CA) for 24 h; T03, -4 ? freezing treatments for 4 h with prior CA; and T04, -4 ? freezing treatments for 4 h without prior CA. In total, 7583 differentially expressed genes (DEGs) of 36462 annotated unigenes were identified. Overall design: Transcriptome of chrysanthemum leaves under four treatments was sequenced by the Illumina sequencing technology. The four treatments are: T01, controls; T02, 4 ? cold acclimation (CA) for 24 h; T03, -4 ? freezing treatments for 4 h with prior CA; and T04, -4 ? freezing treatments for 4 h without prior CA.