FLUTE: a Python GUI for interactive phasor analysis of FLIM data

This repository contains the Fluorescence lifetime imaging microscopy (FLIM) data relative to the following publication "FLUTE: a Python GUI for interactive phasor analysis of FLIM data" https://www.biorxiv.org/content/10.1101/2023.03.31.534529v1 Fluorescein.tif stack contains the fluorescence intensity decay of fluorescein solution with a known lifetime of 4ns, used as calibration. Embryo.tif file contains the fluorescence intensity decay of a zebrafish embryo at 3 days post fertilisation. Both files have been acquired with the following parameters: temporal bin number = 56 laser repetition rates = 80 MHz bin width = 0.223ns