DEG expression in primary human Cytotoxic T Lymphocytes (CTL, CD8+ T-cells) and Natural Killer cells (NK cells) [donor 9 & 2]

Based on differential expression analysis of the transcriptome data and analysis of master regulator genes, we identified 31 candidates which potentially regulate Ca2+-homeostasis in CTL Genes showing differential expression between NK cells and CTL were intersected with members of known Ca2+-signaling pathways and combined with putative key regulators Primary CD8+ T-cells and NK cells were bead-isloated (untouched) and CD8+ T-cells were stimulated with SEA or anti-CD3/CD28 beads, of each template, we analyzed 11 different donors, PBMC were purified from leukocyte reduction chambers (LRS) of healthy platelet donors