Comprehensive transcriptome analysis of the skin in epidermis-specific Nampt cKO mice.

Nicotinamide adenine dinucleotide (NAD?) is essential for cellular metabolism, DNA repair, and stress response. NAD+ is synthesized from nicotinamide, nicotinic acid (collectively termed niacin), and tryptophan. In humans, deficiencies in these nutrients result in pellagra, marked by dermatitis, diarrhea, and dementia. The dermatitis associated with pellagra typically manifests as photodermatosis in sun-exposed areas. This study examined the effects of NAD+ deficiency on skin homeostasis using epidermis-specific Nampt conditional knockout (cKO) mice. These mice displayed substantial NAD? depletion, reduced poly (ADP-ribose) polymerase (PARP) activity, and increased DNA damage, indicated by ?H2AX accumulation. Nampt cKO mice also developed spontaneous skin inflammation and epidermal hyperplasia. Here, we provide gene expression profiling of skin tissues using the RNA sequencing for identifying changes in gene expression of Nampt-cKO mice. Overall design: The mRNA profile of skin tissues was generated by RNA sequencing using the Illumina NovaSeq 6000 (Illumina).