Genomewide poly(A) site mapping for YJR141W and related mutants

Genomewide characterization of YJR141W role in mRNA polyadenylation. Poly(A) site usage was quantified using the 3'T-fill aproach for 3 mutants and a control (BY4741) S. cerevisiae strain. Biological duplicates were analyzed for tsa685 (pcf11-2::KanR; his3^1 leu2^0 ura3^0 met15^0 pcf11-2 BY4741) and tsa801 (CFT2-1::KanR; his3^1 leu2^0 ura3^0 met15^0 cft2-1 BY4741). Only one replicate was analyzed for tsa1248 (YJR141W-PH::KanR; his3^1leu2^0 ura3^0 met15^0 yjr141w-ph BY4741) and BY4741 (Mata; his3^1leu2^0 ura3^0 met15^0). A second batch of libraries (marked as "B") the same starting RNA was performed. The second batch contains biological triplicates for BY4741 and tsa801; and biological duplicates for tsa685 and tsa1248. Samples sharing the same biological origin are labeled with the same roman numeral.