Effects of temperature, pH, and co-solvents on recombinant laccases.

aTm was defined as the temperature that 30-minute incubation led to 50% loss of enzyme activity. Assays were performed in 1×McIlvanie buffer (pH 4) by using 1 mM ABTS at 30°C (Fig. S3).bt1/2,50C was defined as the incubation time at 50°C that caused 50% loss of enzyme activity. Assays were performed in 1×McIlvanie buffer (pH 4) by using 1 mM ABTS at 30°C (Fig. S3).cDefined by residual activity ≥80%. Assays were performed in 1×McIlvanie buffer (pH 2–8) by using 1 mM ABTS at 30°C (Fig. S6).dHalf maximal inhibitory concentration (IC50) was defined as the concentration of co-solvent that caused 50% loss of enzyme activity. Assays were performed in 1×McIlvanie buffer (pH 4) by using 1 mM ABTS at 30°C in the presence of respective solvent (Fig. S4).