Transcriptomic characterization of murine mammary cancer stem cell population

To identify molecular actors of cancer stem cells, we used a double-transgenic mouse model which develop mammary tumors with GFP expression in cancer stem cells. A cell line was generated from tumors of double-transgenic. In this population, GFP+ and GFP- cells was sorted. To identify specific gene expression in GFP+ cells, we performed a microarray analysis Sort was performed 2 days post-seeding. Four independent experiments were performed with cells at 4 different passages.